共同研究報告書


研究区分 開拓型研究

研究課題

 Elucidating psychrophilic algae-bacteria interaction through omics analyses
新規・継続の別 開拓型（2年目/全3年）
研究代表者／所属 北大低温研
研究代表者／職名 助教
研究代表者／氏名 寺島美亜

研究分担者／氏名／所属／職名
　
氏　　名
所　　属
職　　名

1

 高須賀太一 北大農学研究院 助教

2

 堀千明 北大工学研究院 助教

3

 森章一 北大低温研 技術職員

4

 小島久弥 北大低温研 助教

研究目的 This research aims to profile the microbial community of snow in Asahidake and to understand the algae-bacteria interactions of this community. Algae are primary producers in alpine snow， shifting between growth and dormancy， but the regulation between these states are unknown. During growth， algae are green-colored， while dormant algae are red， resulting in green and red snow. Furthermore， bacteria are thought to interact closely with algae. In order to elucidate these interactions， microbial communities of green and red snow will be compared， along with algae and bacteria isolation.
　　
研究内容・成果 Psychrophilic algae blooms can be observed coloring the snow during the melt season in alpine snowfields. These algae are important primary producers on the snow surface environment, supporting the microbial community that coexists with algae, which includes heterotrophic bacteria and fungi. In this study, we analyzed the microbial community of green and red-colored snow containing algae from Mount Asahi, Japan. We found that Chloromonas spp. are the dominant algae in all samples analyzed, and Chlamydomonas is the second-most abundant genus in the red snow. For the bacterial community profile, species belonging to the subphylum Betaproteobacteria were frequently detected in both green and red snow, while members of the phylum Bacteroidetes were also prominent in red snow. Furthermore, multiple independently obtained strains of Chloromonas sp. from inoculates of red snow resulted in the growth of Betaproteobacteria with the alga and the presence of bacteria appears to support growth of the xenic algal cultures under laboratory conditions. The dominance of Betaproteobacteria in algae-containing snow in combination with the detection of Chloromonas sp. with Betaproteobacteria strains suggest that these bacteria can utilize the available carbon source in algae-rich environments and may in turn promote algal growth.
　　
成果となる論文・学会発表等 Publication:
Mia Terashima, Kazuhiro Umezawa, Shoichi Mori, Hisaya Kojima and Manabu Fukui. Microbial community structure analysis of colored snow from alpine snowfield in northern Japan reveals the prevalence of Betaproteobacteria with snow algae. Frontiers in Microbiology 8: 1481. doi:10.3389/fmicb.2017.01481